Insulin-ELISA Kit
Product overview
- Catalog Number
- PPH08-0631
- Sequence
- -
- Target
- Measure & quantify insulin levels in Metabolism & Endocrine research.
- Description
- The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine. Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum and HAMECs (human adipose microvascular endothelial cells) which were isolated from human visceral fat of nondiabetic patients. To measure the blood levels of human insulin and proinsulin in the transgenic mice. To measure serum insulin indaibetic rats. To measure insulin in Multiple Sclerosis patients. Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Insulin and Proinsulin in serum, plasma, cell culture supernatants. This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. INS|IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin.
Category
Product Properties
Quantity | 1mg |
---|---|
Storage | Shipped at 4°C. Store at -20°C for one year. |
Purity | > 95% |
Source | Synthetic |
Forma appearance | Lyophilized powder |
Price | €480,00 |
- For research use only
- Not for use in diagnostic procedures
€480,00 Tax excluded
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