Anti Insulin (Human) Serum (50 μg vial)
Product overview
- Catalog Number
- PPH08-0150
- Sequence
- -
- Target
- This AIRA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AIRA. This ELISA kit for research use only!
- Description
- AIRA ELISA kit applies the competitive enzyme immunoassay technique utilizing Insulin Receptor antigen and an AIRA-HRP conjugate. The assay sample and buffer are incubated together with AIRA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AIRA concentration since AIRA from samples and AIRA-HRP conjugate compete for the Insulin Receptor antigen binding site. Since the number of sites is limited, as more sites are occupied by AIRA from the sample, fewer sites are left to bind AIRA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AIRA concentration in each sample is interpolated from this standard curve.
Category
Product Properties
| Quantity | 1mg |
|---|---|
| Storage | Shipped at 4°C. Store at -20°C for one year. |
| Purity | > 95% |
| Source | Synthetic |
| Forma appearance | Lyophilized powder |
| Price | €408.00 |
- For research use only
- Not for use in diagnostic procedures
€408,00 Tax excluded
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