Azido-PEG3-FLAG (Bulk)

Product overview
Catalog Number
PPH08-0178
Sequence
N3PEG3-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Target
For the Attachment of the Flag Tag by Click Chemistry
Description
Azide-PEG3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne- or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively. The resulting FLAG-tagged molecules can subsequently be detected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solubility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 μM)[1]. This concentrations may serve as a starting point for individual assay set-up.
Category
Catalog peptides, Biologically Active Peptides
Product Properties
Quantity

2mg
Storage

Shipped at 4°C. Store at -20°C for one year.
Purity

> 95%
Source

Synthetic
Forma appearance

Lyophilized powder
Price

€130.00
  • For research use only
  • Not for use in diagnostic procedures

€130,00 Tax excluded
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