HIV-1 TAT Recombinant, Biotin Labeled
Product overview
- Catalog Number
- PPH08-0596
- Sequence
- -
- Target
- Viral Antigens; HIV. Western Blotting and ELISA analysis using streptavidin–HRP conjugated as a detection reagent. Free biotin is eliminated by dialysis against PBS. Protein concentration is determined using a BIORAD protein assay.
- Description
- HIV-1 TAT Biotin Recombinant- produced in E.coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids encoded by two exons and having chain having a molecular mass of 14kDa and was biotinylated with NHS biotin.; 1xPBS; 0.05% glycerol. Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication and infectivity. The HIV-1 TAT is a molecule of 86 aminoacids encoded by two exons. The product of the first exon is sufficient for the transactivation of the HIV-1 promoter. Biotin molecules can be coupled easily to either antigens (such as avidin, streptavidin, Fc binding proteins like Protein A or G) or antibodies and they can be used in a variety of detection systems. Biotin can be conjugated to many proteins without significantly changing their biological activity. Biotin binds with high affinity to avidin, streptavidin and NeutrAvidin protein. Every single protein can react with several molecules of biotin, each of which, in turn, can bind a molecule of avidin.
Category
Product Properties
| Quantity | 1µg, 5µg, 50µg |
|---|---|
| Storage | Shipped at 4°C. Store at -20°C for one year. |
| Purity | > 95% |
| Source | Synthetic |
| Forma appearance | Lyophilized powder |
- For research use only
- Not for use in diagnostic procedures
€30,00 – €980,00 Tax excluded
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