NPY (Human) Antiserum (50 μl vial)

Product overview
Catalog Number
PPH08-0803
Sequence
-
Target
Our undiluted antisera are suitable for immuno-histochemical use, for application to radioimmunoassay (RIA), or other non-isotopicimmunoassay systems, These antisera are partially characterized by either of the following two methods: 1) (EIA) or 2) ELISA
Description
Neuropeptide Y/NPY in Human Brain. The specificity of the NPY antiserum was determined by radioimmunoassay. Synthetic peptides, somatostatin-14, leu-enkephalin, met-enkephalin, oxytocin, Arg8-vasopressin, vasoactive intestinal peptide, angiotensin II demonstrated less than 0.005% cross- reactivity and substance P less than 0.02%. Peptide YY and bovine pancreatic polypeptide demonstrated approx. 36% and 0.05% cross- reactivity respectively. Human peptide YY share 65% homology amino acid sequence for human NPY. Synthetic NPY completely abolished radio labelled NPY binding to NPY antibody in RIA and antibody binding to tissue antigen. Neuropeptide Y/NPY was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human Neuropeptide Y/NPY Monoclonal Antibody (Catalog # MAB8517) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes.
Category
Product Properties
Quantity

1mg

Storage

Shipped at 4°C. Store at -20°C for one year.

Purity

> 95%

Source

Synthetic

Forma appearance

Lyophilized powder

Price

€398,00

  • For research use only
  • Not for use in diagnostic procedures

398,00 Tax excluded

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