Purotoxin-1 (0.1 mg vial)
Product overview
- Catalog Number
- PPH08-0924
- Sequence
- Gly-Tyr-Cys-Ala-Glu-Lys-Gly-Ile-Arg-Cys-Asp-Asp-Ile-His-Cys-Cys-Thr-Gly-Leu-Lys-Cys-Lys-Cys-Asn-Ala-Ser-Gly-Tyr-Asn-Cys-Val-Cys-Arg-Lys-Lys
- Target
- Inhibitor of P2X3 Purinoreceptors, synthetic spider toxin peptide
- Description
- Purotoxin-1 (PT-1) is a peptide originally isolated from the Central Asian spider Geolycosa sp. Purotoxin-1 was shown to inhibit selectively P2X3 receptor channels at a 100 nM concentration. Studies were carried-out on cultured rat DRG neurons. Patch-clamp experiments did not show any inhibitory effect of PT-1 on voltage-gated channels (potentials range tested from -100 to 20 mV), neither on TRPV1 (after activation with 500 nM capsaicin). The selectivity of Purotoxin-1 for P2X3 was highlighted by activating this receptor with 10 µM ATP and 100 µM α, β Methylene-ATP. Indeed, unlike P2X3, P2X2 and heterodimer P2X2/3 are known to be not sensitive to such concentrations. Moreover, P2X3, P2X2, and P2X2/3 are the only known ATP-sensitive receptors expressed in plasma membranes of DRG neurons. So, the observed effect seems to be well related to a selective inhibition of P2X3. P2X3-mediated current was fully inhibited with 100 nM Purotoxin-1, making it the most potent and selective ligand for P2X3.
Category
Product Properties
| Quantity | 1mg |
|---|---|
| Storage | Shipped at 4°C. Store at -20°C for one year. |
| Purity | > 95% |
| Source | Synthetic |
| Forma appearance | Lyophilized powder |
| Price | €230,00 |
- For research use only
- Not for use in diagnostic procedures
€230,00 Tax excluded
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